• 4 months ago
Transcript
00:00:00you
00:00:30you
00:02:31course
00:02:37course
00:02:40cross link
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00:03:04method
00:03:37So, glycoprotein and lipid, the main thing is that glycoprotein and glycolipid, the two
00:03:57things together created the sugar level.
00:03:59The sugar level created by these two things together was glycocalyx.
00:04:04Okay?
00:04:05Okay, fine.
00:04:06So, we are generally seeing that you have some memories.
00:04:09Which I said again, if you do not remember, there is no problem.
00:04:12But, recap a little bit, because now you are starting the study again.
00:04:16So, keep the focus and start with concentration.
00:04:19So, let's go a little earlier.
00:04:21Today's B3 lecture will start.
00:04:23Okay, we are talking about glycoprotein.
00:04:26Okay, we will get into the topic.
00:04:28Before that, let's go.
00:04:29We are a little bit, suppose, with the small light of Doraemon, if we go completely crazy,
00:04:34then somehow let's see.
00:04:36So, what are we doing completely crazy?
00:04:38We are going to explore the cell.
00:04:40So, you went slowly, slowly, in front of a cell.
00:04:44Suppose, it is a plant cell.
00:04:46So, what will happen?
00:04:47What will we see outside everyone?
00:04:49So, inside the cell, there is an old layer, which is called the old cell.
00:04:53After that, if we go inside the old cell, what will we get?
00:04:56The old skin.
00:04:57Right?
00:04:58Then, what will we get?
00:04:59Cytoplasm.
00:05:00In cytoplasm, there are different organs and others.
00:05:02Right?
00:05:03So, in this, one organ is called the brain of the cell.
00:05:06So, you guess a little bit, who can be called the brain of the cell?
00:05:10Haan.
00:05:11Nucleus.
00:05:12Yup.
00:05:43So, this is the membrane of the nucleus, and this is the membrane of the nucleus, and this
00:05:58is the membrane of the nucleus, and this is the membrane of the nucleus, and this is the
00:06:07membrane of the nucleus, and this is the membrane of the nucleus, and this is the membrane
00:06:14of the nucleus, and this is the membrane of the nucleus, and this is the membrane of the
00:06:21nucleus, and this is the membrane of the nucleus, and this is the membrane of the nucleus, and
00:06:28this is the membrane of the nucleus, and this is the membrane of the nucleus, and this is
00:06:35the membrane of the nucleus, and this is the membrane of the nucleus, and this is the membrane
00:06:42of the nucleus, and this is the membrane of the nucleus, and this is the membrane of the
00:06:49nucleus, and this is the membrane of the nucleus, and this is the membrane of the nucleus, and
00:06:54this is the membrane of the nucleus, and this is the membrane of the nucleus, and this is
00:07:01the membrane of the nucleus, and this is the membrane of the nucleus, and this is the membrane
00:07:08of the nucleus, and this is the membrane of the nucleus, and this is the membrane of the
00:07:15nucleus, and this is the membrane of the nucleus, and this is the membrane of the nucleus, and
00:07:20this is the membrane of the nucleus, and this is the membrane of the nucleus, and this is
00:07:28the membrane of the nucleus, and this is the membrane of the nucleus, and this is the membrane
00:07:40of the nucleus, and this is the membrane of the nucleus, and this is the membrane of the
00:07:47nucleus, and this is the membrane of the nucleus.
00:08:17DNA to RNA, RNA comes from ribosome and makes protein, we have already learnt about it.
00:08:25So, can you see, there is another type of ribosome around this ribosome, or in some cases,
00:08:29it is rotating freely, and in some cases, it is attached to the endoplasmic reticulum like this.
00:08:41So, this is how it is attached to the endoplasmic reticulum.
00:08:47Now, if I tell you, I am not going to give you the whole picture of the endoplasmic reticulum.
00:08:54See, what I have given in the first part.
00:08:57Normally, it is like this.
00:08:58You can see, I am showing you two stages.
00:09:01This is the first stage, in which the endoplasmic reticulum is attached.
00:09:07So, what will happen?
00:09:08Now, it will become a little rough.
00:09:10It will not be as muscular as a lizard.
00:09:13So, this is why it is called the amosrine endoplasmic reticulum.
00:09:20Amosrine endoplasmic reticulum.
00:09:22And the part after this, have you seen any ribosome?
00:09:24No, but this part we are talking about is the amosrine endoplasmic reticulum.
00:09:31Now, we have read three objectives.
00:09:34If the first question is asked,
00:09:36Tell me, what is the origin of the endoplasmic reticulum?
00:09:42Tell me, what is the origin of the endoplasmic reticulum?
00:09:49Of course, you will not say, what is this?
00:09:51Nucleus membrane or nuclear membrane.
00:09:54Not nucleus.
00:09:55Nuclear membrane.
00:09:56But, in some cases, it can be from the core membrane.
00:10:00Rare case.
00:10:01But, in most cases, it can be from the nucleus membrane.
00:10:04First objective done.
00:10:05Now, we are looking at two types of endoplasmic reticulum.
00:10:09One is the amosrine and the other is the mosrine.
00:10:12What is the amosrine?
00:10:13In many cases, the ribosome is visible.
00:10:15And what is the mosrine?
00:10:16Nothing.
00:10:17Now, if I ask the question,
00:10:19Tell me, what is the work of the amosrine endoplasmic reticulum?
00:10:24Can you tell me?
00:10:25You can see the picture.
00:10:26What is the work of the amosrine endoplasmic reticulum?
00:10:29Tell me.
00:10:31What is the work of the amosrine endoplasmic reticulum?
00:10:35What can happen?
00:10:36I hope you can.
00:10:37Yes, protein is made.
00:10:39Isn't it?
00:10:40Yes.
00:10:41Then, I will see, what is the work of the amosrine?
00:10:45This work is to make protein.
00:10:49Then, what is the work of the mosrine?
00:10:53Keep in mind.
00:10:54After making the protein, everything else is made by the mosrine.
00:10:59That is, everything else.
00:11:01What does everything else mean?
00:11:03For example, we know carbohydrate, protein and lipid.
00:11:06Then, protein is made.
00:11:07The rest is carbohydrate.
00:11:09So, in carbohydrate, there is glycogen.
00:11:12Then, there is lipid.
00:11:15Then, there is hormone.
00:11:17Is it right?
00:11:18Mosrine endoplasmic reticulum makes all these things.
00:11:23Now, the question is,
00:11:25Then, what is the work of the ribosome?
00:11:27What is the work of the ribosome with this ribosome?
00:11:29Let's see.
00:11:30For example,
00:11:31We have heard the name of enzymes.
00:11:34Enzyme is a kind of protein.
00:11:36Now, from this ribosome,
00:11:38After going to the mRNA ribosome,
00:11:40What is made,
00:11:41This can be called the raw stage.
00:11:43Raw stage means,
00:11:44For example,
00:11:45We used to eat potato chips.
00:11:47Now, this is made from potatoes.
00:11:50Now, they are processing, cutting, roasting, and other things.
00:11:56After that,
00:11:57Packaging is done.
00:11:58And you get it.
00:11:59Isn't it?
00:12:00Now,
00:12:01We are not eating normal raw potato.
00:12:03We are eating it in a modified state.
00:12:05So, this raw potato,
00:12:06The ribosome that makes the protein,
00:12:08Is it right?
00:12:09Now,
00:12:10The ribosome that makes the protein,
00:12:13They release the same protein in this place.
00:12:16That is,
00:12:17Inside the endoplasmic reticulum.
00:12:20Is it right?
00:12:21That is,
00:12:22The protein that comes from their body.
00:12:25Do you understand?
00:12:27So,
00:12:28The protein that comes from their mouth,
00:12:31The protein that they make,
00:12:33They release it in the cytoplasm.
00:12:36That is,
00:12:37They release it in the cytoplasm.
00:12:39Clear?
00:12:40But, how do they send it inside the endoplasmic reticulum?
00:12:44In this state.
00:12:45But, I was saying one thing.
00:12:47What is this?
00:12:48This is not the final stage.
00:12:50This is the raw stage.
00:12:51Now,
00:12:52What does the muscle do?
00:12:53The muscle makes glycogen,
00:12:55Glycoprotein,
00:12:56Lipids,
00:12:57Hormones,
00:12:58And others.
00:12:59It makes a lot of things.
00:13:01But,
00:13:02I am saying again,
00:13:03What is this stage?
00:13:04This is the preliminary stage,
00:13:05The raw stage,
00:13:06Whatever you say.
00:13:07Is it right?
00:13:08But,
00:13:09The protein is the final stage.
00:13:11Is it right?
00:13:12Ok,
00:13:13I don't understand.
00:13:14We will come,
00:13:15No problem.
00:13:16We will come again.
00:13:17When the whole thing is clear,
00:13:18Then,
00:13:19The whole thing will be understood.
00:13:20Is it right?
00:13:21What I said,
00:13:22Now,
00:13:23I am trying to make chips.
00:13:24So,
00:13:25From endoplasmic reticulum,
00:13:26Whatever is created,
00:13:27Protein is created from the muscle,
00:13:28From the muscle,
00:13:29Glycogen,
00:13:30Lipids,
00:13:31Hormones,
00:13:32And others.
00:13:33All these are the preliminary states.
00:13:34Now,
00:13:35This preliminary state,
00:13:36Must be finalized,
00:13:37Must be modified,
00:13:38Must be packed.
00:13:39So,
00:13:40Let's see,
00:13:41What happens?
00:13:42So,
00:13:43What happens?
00:13:44So,
00:13:45What happens?
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00:33:00So,
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00:34:00So,
00:34:01So,
00:34:02So,
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00:35:00So,
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00:35:02So,
00:35:03So,
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00:36:00So,
00:36:01So,
00:36:02So,
00:36:03So,
00:36:04So,
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00:36:06So,
00:36:07So,
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00:36:10So,
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00:36:19So,
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00:36:34So,
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00:36:40So,
00:36:41So,
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00:36:43So,
00:36:44So,
00:36:45So,
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00:36:47So,
00:36:48So,
00:36:49So,
00:36:50So,
00:36:51So,
00:36:52So,
00:36:53So,
00:36:54So,
00:36:55So,
00:36:56So,
00:36:57So,
00:36:58So,
00:36:59So,
00:37:00So,
00:37:01So,
00:37:02So,
00:37:03So,
00:37:04So,
00:37:05So,
00:37:06So,
00:37:07So,
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00:37:09So,
00:37:10So,
00:37:11So,
00:37:12So,
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00:37:15So,
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00:37:17So,
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00:37:19So,
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00:37:21So,
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00:37:24So,
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00:37:29So,
00:37:30So,
00:37:31So,
00:37:32So,
00:37:33So,
00:37:34So,
00:37:35So,
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00:37:37So,
00:37:38So,
00:37:39So,
00:37:40So,
00:37:41So,
00:37:42So,
00:37:43So,
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00:37:45So,
00:37:46So,
00:37:47So,
00:37:48So,
00:37:49So,
00:37:50So,
00:37:51So,
00:37:52So,
00:37:53So,
00:37:54So,
00:37:55So,
00:37:56So,
00:37:57So,
00:37:58So,
00:37:59So,
00:38:00So,
00:38:01So,
00:38:02So,
00:38:03So,
00:38:04So,
00:38:05So,
00:38:06So,
00:38:07So,
00:38:08So,
00:38:09So,
00:38:10So,
00:38:11So,
00:38:12So,
00:38:13So,
00:38:14So,
00:38:15So,
00:38:16So,
00:38:17So,
00:38:18So,
00:38:19So,
00:38:20So,
00:38:21So,
00:38:22So,
00:38:23So,
00:38:24So,
00:38:25So,
00:38:26So,
00:38:27So,
00:38:28So,
00:38:29So,
00:38:30So,
00:38:31So,
00:38:32So,
00:38:33So,
00:38:34So,
00:38:35So,
00:38:36So,
00:38:37So,
00:38:38So,
00:38:39So,
00:38:40So,
00:38:41So,
00:38:42So,
00:38:43So,
00:38:44So,
00:38:45So,
00:38:46So,
00:38:47So,
00:38:48So,
00:38:49So,
00:38:50So,
00:38:51So,
00:38:52So,
00:38:53So,
00:38:54So,
00:38:55So,
00:38:56So,
00:38:57So,
00:38:58So,
00:38:59So,
00:39:00So,
00:39:01So,
00:39:02So,
00:39:03So,
00:39:04So,
00:39:05So,
00:39:06So,
00:39:07So,
00:39:08So,
00:39:09So,
00:39:10So,
00:39:11So,
00:39:12So,
00:39:13So,
00:39:14So,
00:39:15So,
00:39:16So,
00:39:17So,
00:39:18So,
00:39:19So,
00:39:20So,
00:39:21So,
00:39:22So,
00:39:23So,
00:39:24So,
00:39:25So,
00:39:26So,
00:39:27So,
00:39:28So,
00:39:29So,
00:39:30So,
00:39:31So,
00:39:32So,
00:39:33So,
00:39:34So,
00:39:35So,
00:39:36So,
00:39:37So,
00:39:38So,
00:39:39So,
00:39:40So,
00:39:41So,
00:39:42So,
00:39:43So,
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00:39:48So,
00:39:49So,
00:39:50So,
00:39:51So,
00:39:52So,
00:39:53So,
00:39:54So,
00:39:55So,
00:39:56So,
00:39:57So,
00:39:58So,
00:39:59So,
00:40:00So,
00:40:01So,
00:40:02So,
00:40:03So,
00:40:04So,
00:40:05So,
00:40:06So,
00:40:07So,
00:40:08So,
00:40:09So,
00:40:10So,
00:40:11So,
00:40:12So,
00:40:13So,
00:40:14So,
00:40:15So,
00:40:16So,
00:40:17So,
00:40:18So,
00:40:19So,
00:40:20So,
00:40:21So,
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00:40:24So,
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00:42:00So,
00:42:01So,
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00:42:03So,
00:42:04So,
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00:42:20So,
00:42:21So,
00:42:22So,
00:42:23So,
00:42:24So,
00:42:25So,
00:42:26So,
00:42:27So,
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00:42:29So,
00:42:30So,
00:42:31So,
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00:42:35So,
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00:42:37So,
00:42:38So,
00:42:39So,
00:42:40So,
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00:42:44So,
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00:42:48So,
00:42:49So,
00:42:50So,
00:42:51So,
00:42:52So,
00:42:53So,
00:42:54So,
00:42:55So,
00:42:56So,
00:42:57So,
00:42:58So,
00:42:59So,
00:43:00So,
00:43:01So,
00:43:02So,
00:43:03So,
00:43:04So,
00:43:05So,
00:43:06So,
00:43:07So,
00:43:08So,
00:43:09So,
00:43:10So,
00:43:11So,
00:43:12So,
00:43:13So,
00:43:14So,
00:43:15So,
00:43:16So,
00:43:17So,
00:43:18So,
00:43:19So,
00:43:20So,
00:43:21So,
00:43:22So,
00:43:23So,
00:43:24So,
00:43:25So,
00:43:26So,
00:43:27So,
00:43:28So,
00:43:29So,
00:43:30So,
00:43:31So,
00:43:32So,
00:43:33So,
00:43:34So,
00:43:35So,
00:43:36So,
00:43:37So,
00:43:38So,
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00:43:40So,
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00:43:48So,
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00:43:57So,
00:43:58So,
00:43:59So,
00:44:00So,
00:44:01So,
00:44:02So,
00:44:03So,
00:44:04So,
00:44:05So,
00:44:06So,
00:44:07So,
00:44:08So,
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00:44:23So,
00:44:24So,
00:44:25So,
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00:44:27So,
00:44:28So,
00:44:29So,
00:44:30So,
00:44:31So,
00:44:32So,
00:44:33So,
00:44:34So,
00:44:35So,
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00:44:37So,
00:44:38So,
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00:44:40So,
00:44:41So,
00:44:42So,
00:44:43So,
00:44:44So,
00:44:45So,
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00:44:47So,
00:44:48So,
00:44:49So,
00:44:50So,
00:44:51So,
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00:44:53So,
00:44:54So,
00:44:55So,
00:44:56So,
00:44:57So,
00:44:58So,
00:44:59So,
00:45:00So,
00:45:01So,
00:45:02So,
00:45:03So,
00:45:04So,
00:45:05So,
00:45:06So,
00:45:07So,
00:45:08So,
00:45:09So,
00:45:10So,
00:45:11So,
00:45:12So,
00:45:13So,
00:45:14So,
00:45:15So,
00:45:16So,
00:45:17So,
00:45:18So,
00:45:19So,
00:45:20So,
00:45:21So,
00:45:22So,
00:45:23So,
00:45:24So,
00:45:25So,
00:45:26So,
00:45:27So,
00:45:28So,
00:45:29So,
00:45:30So,
00:45:31So,
00:45:32So,
00:45:33So,
00:45:34So,
00:45:35So,
00:45:36So,
00:45:37So,
00:45:38So,
00:45:39So,
00:45:40So,
00:45:41So,
00:45:42So,
00:45:43So,
00:45:44So,
00:45:45So,
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00:45:48So,
00:45:49So,
00:45:50So,
00:45:51So,
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00:45:53So,
00:45:54So,
00:45:55So,
00:45:56So,
00:45:57So,
00:45:58So,
00:45:59So,
00:46:00So,
00:46:01So,
00:46:02So,
00:46:03So,
00:46:04So,
00:46:05So,
00:46:06So,
00:46:07So,
00:46:08So,
00:46:09So,
00:46:10So,
00:46:11So,
00:46:12So,
00:46:13So,
00:46:14So,
00:46:15So,
00:46:16So,
00:46:17So,
00:46:18So,
00:46:19So,
00:46:20So,
00:46:21So,
00:46:22So,
00:46:23So,
00:46:24So,
00:46:25So,
00:46:26So,
00:46:27So,
00:46:28So,
00:46:29So,
00:46:30So,
00:46:31So,
00:46:32So,
00:46:33So,
00:46:34So,
00:46:35So,
00:46:36So,
00:46:37So,
00:46:38So,
00:46:39So,
00:46:40So,
00:46:41So,
00:46:42So,
00:46:43So,
00:46:44So,
00:46:45So,
00:46:46So,
00:46:47So,
00:46:48So,
00:46:49So,
00:46:50So,
00:46:51So,
00:46:52So,
00:46:53So,
00:46:54So,
00:46:55So,
00:46:56So,
00:46:57So,
00:46:58So,
00:46:59So,
00:47:00So,
00:47:01So,
00:47:02So,
00:47:03So,
00:47:04So,
00:47:05So,
00:47:06So,
00:47:07So,
00:47:08So,
00:47:09So,
00:47:10So,
00:47:11So,
00:47:12So,
00:47:13So,
00:47:14So,
00:47:15So,
00:47:16So,
00:47:17So,
00:47:18So,
00:47:19So,
00:47:20So,
00:47:21So,
00:47:22So,
00:47:23So,
00:47:24So,
00:47:25So,
00:47:26So,
00:47:27So,
00:47:28So,
00:47:29So,
00:47:30So,
00:47:31So,
00:47:32So,
00:47:33So,
00:47:34So,
00:47:35So,
00:47:36So,
00:47:37So,
00:47:38So,
00:47:39So,
00:47:40So,
00:47:41So,
00:47:42So,
00:47:43So,
00:47:44So,
00:47:45So,
00:47:46So,
00:47:47So,
00:47:48So,
00:47:49So,
00:47:50So,
00:47:51So,
00:47:52So,
00:47:53So,
00:47:54So,
00:47:55So,
00:47:56So,
00:47:57So,
00:47:58So,
00:47:59So,
00:48:00So,
00:48:01So,
00:48:02So,
00:48:03So,
00:48:04So,
00:48:05So,
00:48:06So,
00:48:07So,
00:48:08So,
00:48:09So,
00:48:10So,
00:48:11So,
00:48:12So,
00:48:13So,
00:48:14So,
00:48:15So,
00:48:16So,
00:48:17So,
00:48:18So,
00:48:19So,
00:48:20So,
00:48:21So,
00:48:22So,
00:48:23So,
00:48:24So,
00:48:25So,
00:48:26So,
00:48:27So,
00:48:28So,
00:48:29So,
00:48:30So,
00:48:31So,
00:48:32So,
00:48:33So,
00:48:34So,
00:48:35So,
00:48:36So,
00:48:37So,
00:48:38So,
00:48:39So,
00:48:40So,
00:48:41So,
00:48:42So,
00:48:43So,
00:48:44So,
00:48:45So,
00:48:46So,
00:48:47So,
00:48:48So,
00:48:49So,
00:48:50So,
00:48:51So,
00:48:52So,
00:48:53So,
00:48:54So,
00:48:55So,
00:48:56So,
00:48:57So,
00:48:58So,
00:48:59So,
00:49:00So,
00:49:01So,
00:49:02So,
00:49:03So,
00:49:04So,
00:49:05So,
00:49:06So,
00:49:07So,
00:49:08So,
00:49:09So,
00:49:10So,
00:49:11So,
00:49:12So,
00:49:13So,
00:49:14So,
00:49:15So,
00:49:16So,
00:49:17So,
00:49:18So,
00:49:19So,
00:49:20So,
00:49:21So,
00:49:22So,
00:49:23So,
00:49:24So,
00:49:25So,
00:49:26So,
00:49:27So,
00:49:28So,
00:49:29So,
00:49:30So,
00:49:31So,
00:49:32So,
00:49:33So,
00:49:34So,
00:49:35So,
00:49:36So,
00:49:37So,
00:49:38So,
00:49:39So,
00:49:40So,
00:49:41So,
00:49:42So,
00:49:43So,
00:49:44So,
00:49:45So,
00:49:46So,
00:49:47So,
00:49:48So,
00:49:49So,
00:49:50So,
00:49:51So,
00:49:52So,
00:49:53So,
00:49:54So,
00:49:55So,
00:49:56So,
00:49:57So,
00:49:58So,
00:49:59So,
00:50:00So,
00:50:01So,
00:50:02So,
00:50:03So,
00:50:04So,
00:50:05So,
00:50:06So,
00:50:07So,
00:50:08So,
00:50:09So,
00:50:10So,
00:50:11So,
00:50:12So,
00:50:13So,
00:50:14So,
00:50:15So,
00:50:16So,
00:50:17So,
00:50:18So,
00:50:19So,
00:50:20So,
00:50:21So,
00:50:22So,
00:50:23So,
00:50:24So,
00:50:25So,
00:50:26So,
00:50:27So,
00:50:28So,
00:50:29So,
00:50:30So,
00:50:31So,
00:50:32So,
00:50:33So,
00:50:34So,
00:50:35So,
00:50:36So,
00:50:37So,
00:50:38So,
00:50:39So,
00:50:40So,
00:50:41So,
00:50:42So,
00:50:43So,
00:50:44So,
00:50:45So,
00:50:46So,
00:50:47So,
00:50:48So,
00:50:49So,
00:50:50So,
00:50:51So,
00:50:52So,
00:50:53So,
00:50:54So,
00:50:55So,
00:50:56So,
00:50:57So,
00:50:58So,
00:50:59So,
00:51:00So,
00:51:01So,
00:51:02So,
00:51:03So,
00:51:04So,
00:51:05So,
00:51:06So,
00:51:07So,
00:51:08So,
00:51:09So,
00:51:10So,
00:51:11So,
00:51:12So,
00:51:13So,
00:51:14So,
00:51:15So,
00:51:16So,
00:51:17So,
00:51:18So,
00:51:19So,
00:51:20So,
00:51:21So,
00:51:22So,
00:51:23So,
00:51:24So,
00:51:25So,
00:51:26So,
00:51:27So,
00:51:28So,
00:51:29So,
00:51:30So,
00:51:31So,
00:51:32So,
00:51:33So,
00:51:34So,
00:51:35So,
00:51:36So,
00:51:37So,
00:51:38So,
00:51:39So,
00:51:40So,
00:51:41So,
00:51:42So,
00:51:43So,
00:51:44So,
00:51:45So,
00:51:46So,
00:51:47So,
00:51:48So,
00:51:49So,
00:51:50So,
00:51:51So,
00:51:52So,
00:51:53So,
00:51:54So,
00:51:55So,
00:51:56So,
00:51:57So,
00:51:58So,
00:51:59So,
00:52:00So,
00:52:01So,
00:52:02So,
00:52:03So,
00:52:04So,
00:52:05So,
00:52:06So,
00:52:07So,
00:52:08So,
00:52:09So,
00:52:10So,
00:52:11So,
00:52:12So,
00:52:13So,
00:52:14So,
00:52:15So,
00:52:16So,
00:52:17So,
00:52:18So,
00:52:19So,
00:52:20So,
00:52:21So,
00:52:22So,
00:52:23So,
00:52:24So,
00:52:25So,
00:52:26So,
00:52:27So,
00:52:28So,
00:52:29So,
00:52:30So,
00:52:31So,
00:52:32So,
00:52:33So,
00:52:34So,
00:52:35So,
00:52:36So,
00:52:37So,
00:52:38So,
00:52:39So,
00:52:40So,
00:52:41So,
00:52:42So,
00:52:43So,
00:52:44So,
00:52:45So,
00:52:46So,
00:52:47So,
00:52:48So,
00:52:49So,
00:52:50So,
00:52:51So,
00:52:52So,
00:52:53So,
00:52:54So,
00:52:55So,
00:52:56So,
00:52:57So,
00:52:58So,
00:52:59So,
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01:06:57So,
01:06:58So,
01:06:59So,
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